Announcements

SCIENCE SEMINAR by Suna Timur

Author: COLLEGE OF SCIENCES
Time: 17:00
Location: SCI 103

SCIENCE SEMINAR

 

Date      : December 6, 2012

Time      : 17:00

Place     : SCI 103

Refreshments will be served at 16:45

 

Suna TÝMUR

Ege University

Faculty of Science

Biochemistry Department

 

 

“Bio-Functionalized Fluorescence Probes: Cell Specific Targeting and Imaging”

 

 

Abstract:

The development of nanotechnology provides us new functionalized fluorescent nanoparticles, quantum dots (QDs), as probes for biomedical applications it will bring new alternates to cancer researches. As fluorescent probes, QDs have many unique characteristics. QDs have different emission wavelengths depending on their sizes and also, they have bio-conjugation ability with various bio-molecules, effectively. Thus, all of these features make QDs very suitable materials for bio-labeling, targeting and functionalization studies. In our studies, various QDs have been used for the bio-functionalization as platform for cancer cell targeting and imaging studies. Initially, phase-transfer reaction of QDs with amine-terminated polyamidoamine (PAMAM) dendrimer with controllable ligand molar ratios was achieved. After conjugation with HER-2, receptor-mediated targeting efficiency of antibody labeled PAMAM/QDs conjugates was evaluated by successful staining of breast cancer cells (MCF-7) with bio-conjugates. Moreover, carboxyl functional QDs have been targeted with anti-c-erbB2 antibody via EDC/NHS chemistry for the imaging of lung cancer cells (A-549) and then in vitro studies were carried out with A-549 cells to observe their cell internalization efficiency. Finally, PEGylated QDs were modified with cell penetrating peptide that facilitates penetration of QDs from the cell membrane. Cellular internalization degree and the localization of bio-conjugates were observed via fluorescence properties of QDs using fluorescence microscopy and flow cytometry. Our findings confirmed that designed bio-functionalized QD nanoparticles have great potential in the area of targeted cellular imaging and therapy.