MBGE SEMINAR by Dr. OgŁn Adebali

Time: 10:30
Location: SCI 103


Speaker                 : Dr. Ogün Adebali, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill


Title                    :  Pinpointing DNA damage, repair and the effect of a mutation


Date                    : June 20, Tuesday

Time                   : 10:30 am

Cookie & Tea        : 10:15 am SCI 103  

Place                   : SCI 103


Abstract             : Our DNA is continually damaged by cellular and environmental factors. Some of the most common mutagens, including UV light and platinum-based drugs, cause bulky adduct formation; these are almost exclusively corrected through nucleotide excision repair. Although the basic mechanisms of excision repair are reasonably well understood, we previously did not know which genomic loci are preferentially damaged and repaired. Using the novel technologies, Damage-seq and XR-seq, we were able to decipher the genome-wide kinetics and location of damage formation and excision repair following ultraviolet irradiation and cisplatin treatments. These single-nucleotide resolution maps revealed cellular components affecting the DNA damage formation and repair. The damages that escape the repair machinery can result in mutagenesis. In clinics, there are currently no accurate ways to determine the consequences of a novel mutation. We propose a new computational perspective to predicting pathogenicity of single point mutations by building the precise evolutionary histories of genes. Taken together, we apply genomic technologies to understand how DNA is damaged and repaired, as well as whether unrepaired mutagenic damages lead to pathogenicity or not.