Join Zoom Meeting-MBGE SEMINAR by Ali Akhtar

Time: 11:00
Location: Zoom meeting



Speaker          : Ali Akhtar, Ph.D., Konkuk University Seoul, South Korea

Title                 : HOS15 negatively regulates ABA signaling and drought stress in plants via SnRK2s degradation

Date                : Tuesday, November 24, 2020

Time                : 11:00-12:00

Zoom info      : Join Zoom Meeting


Meeting ID: 990 8589 6798

Passcode: 869964



Dehydrating stresses trigger the accumulation of abscisic acid (ABA), a key plant stress-signaling hormone that activates Snf1-Related Kinases (SnRK2s) to mount adaptive responses. However, the regulatory circuits that terminate the SnRK2s signal relay after acclimation or post-stress conditions remain to be defined. Here, I show that the desensitization of the ABA-signal is achieved by the regulation of OST1 (SnRK2.6) protein stability via the E3-ubiquitin-ligase HOS15. Upon ABA signal, HOS15-induced degradation of OST1 is inhibited and stabilized OST1 promotes the stress-response. When the ABA signal terminates, protein phosphatases ABI1/2 promote the rapid degradation of OST1 via HOS15. Notably, we found that even in the presence of ABA, OST1 levels were also depleted within hours of ABA signal onset. The unexpected dynamics of OST1 abundance was resolved by a systematic mathematical modeling demonstrating a desensitizing feedback loop by which OST1-induced up-regulation of ABI1/2 leads to the degradation of OST1. This model illustrates the complex rheostat dynamics underlying the ABA-induced stress response and desensitization.

Key words: ABA-signaling, Drought stress, HOS15, OST1, ABI1/2, Protein degradation and stability.